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TAK-243 preferentially certain to UBA1 above the connected E1 enzymes UBA2, UBA3, and UBA6 in intact AML cells. Inhibition of UBA1 with TAK-243 lowered levels of ubiquitylated proteins, increased markers of proteotoxic tension and DNA injury worry. In vivo, TAK-243 reduced leukemic stress and focused leukemic stem cells without having evidence of t